This document presents the SPHINX use cases that specifically address users' requirements and expectations on the use of new and advanced cybersecurity tools and services for the benefit of healthcare organisations and their patients. In particular, this document provides an overview and the main outcomes of the work performed by the SPHINX Consortium on the SPHINX use cases, as part of Task 2.4 – Reference Scenarios and Pilot Operations Specifications and KPIs for a period of two months (month

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... 23 or November 2020 and month 24 or December 2020). Based on a compound framework of critical assets, threat taxonomy and actors, attack vectors and impact, this document updates deliverable D2.4 with five new use cases (use cases 17 to 21) that enrich the set of SPHINX use cases forming a basis for the three SPHINX pilots, as well as helping with the definition of the SPHINX user requirements (WP2), technical specifications (WP2) and development effort (WPs 3 to 6), while supporting SPHINX testing and validation effort (WP7). Overall, the SPHINX use cases are intended to highlight the greatest possible breadth of the SPHINX's capabilities, whilst remaining realistic and testable in realistic conditions. They also seek, where possible, to address potential risks and limitations of the SPHINX System and its usage. In addition, this report discloses the relevant work SPHINX partners have been developing to support the SPHINX piloting activities, namely in what concerns the key performance indicators identified as relevant to enable the evaluation of the SPHINX's impact in cybersecurity systems and healthcare organisations.