Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil

Ana Lys Bezerra Barrradas Mineiro, Rômulo José Vieira, Érica Azevedo Costa, Renato Lima Santos, Larissa Maria Feitosa Gonçalves, Sônia Maria Carvalho, Maria Rosa Quaresma Bomfim, Francisco Assis Lima Costa
2011 Pesquisa Veterinária Brasileira  
The presence of anti leptospiral agglutinins (microscopic agglutination test - MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction - PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent
more » ... ls for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman's capsule of 20 positive animals. Inflammatory cells were more common in the kidney. PCR was positive in urine and kidney tissue
doi:10.1590/s0100-736x2011001000005 fatcat:zk3x73k6jrazvblchmzte6prva