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Silybum marianum (L) Gaertn an important Indian medicinal herb has evolved wide interest for its silymarin (silybin) content, which is emerging as a very useful molecule. The callus culture was induced from leaf explant on MS medium supplemented with various growth regulators for the in vitro production of silybin. The best hormonal combination found was IAA + IBA + 2,4D and kinetin (1 ppm each). Growth kinet-ics of callus culture was studied using fresh weight parameter and results has shownfatcat:kyszn2ysenhwvf77ac2hynigvi