Engineering stable topography in dense bio-mimetic 3D collagen scaffolds

T Alekseeva, E Hadjipanayi, EA Abou Neel, RA Brown
2012 European Cells and Materials  
Topographic features are well known to infl uence cell behaviour and can provide a powerful tool for engineering complex, functional tissues. This study aimed to investigate the mechanisms of formation of a stable micro-topography on plastic compressed (PC) collagen gels. The unidirectional fl uid fl ow that accompanies PC of collagen gels creates a fl uid leaving surface (FLS) and a non-fl uid leaving surface (non-FLS). Here we tested the hypothesis that the resulting anisotropy in collagen
more » ... sity and stiffness between FLS and non-FLS would infl uence the fi delity and stability of micro-grooves patterned on these surfaces. A pattern template of parallel-aligned glass fi bres was introduced to the FLS or non-FLS either at the start of the compression or halfway through, when a dense FLS had already formed. Results showed that both early and late patterning of the FLS generated grooves that had depth (25 ±7 μm and 19 ±8 μm, respectively) and width (55 ±11 μm and 50 ±12 μm, respectively) which matched the glass fi bre diameter (50 μm). In contrast, early and late patterning of the non-FLS gave much wider (151 ±50 μm and 89 ±14 μm, respectively) and shallower (10 ±2.7 μm and 13 ±3.5 μm, respectively) grooves than expected. The depth to width ratio of the grooves generated on the FLS remained unaltered under static culture conditions over 2 weeks, indicating that grooves were stable under long term active cell-mediated matrix remodelling. These results indicate that the FLS, characterised by a higher matrix collagen density and stiffness than the non-FLS, provides the most favourable mechanical surface for precise engineering of a stable micro-topography in 3D collagen hydrogel scaffolds.
doi:10.22203/ecm.v023a03 fatcat:aakoeiymifhehmhgpnolfk6opi