AGRADECIMENTOS Aos meus pais, Brenda e Javier, que sempre me apoiaram, são meus exemplos de vida e me forneceram o que foi necessário para que conseguisse alcançar meus objetivos. Aos meus amigos e professores Carla B. Belli, Luís Claudio Lopes Correa da Silva, André L.V. de Zoppa, Fabio Celidonio Plogliani. Às minhas duas casas de coração FMVZ-Unillanos-Colômbia e FMVZ-USP, por toda a base, inúmeros ensinamentos, profissionais e pessoais, e bons momentos vividos ao lado de pessoas especiais

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... conheci nessa jornada. À minha orientadora Raquel Yvonne Arantes Baccarin, que me acolheu como orientado estando sempre presente para auxiliar no projeto, nas burocracias necessárias e que mesmo sem financiamento fez com que sua realização fosse possível. Ao meu coorientador Rodrigo Romero Correa e sua esposa Lia que me acolheram e me deram auxílio durante a realização do projeto, pelas risadas e bons momentos, deixando uma experiência inesquecível. Ao professor Bruno Cogliati, pela ajuda com a histopatologia e as análises das biopsias. Ao meu colega Diego Velasquez, pelo auxílio durante a realização do projeto, pelas risadas e bons momentos, deixando o fardo muito mais leve. Aos meus companheiros de pós-graduação, Natalia, Murillo, Jean, Joice, Sarah, Fernanda, Cynthia e Eric que me ajudaram durante essa árdua jornada, no cuidado com os cavalos, nas coletas e análises. Aos meus amigos e irmãos Yenny , pelo apoio e força para lutar por meus objetivos e sonhos sempre. Aos enfermeiros Marquinhos, Cícero e Gerson, por toda ajuda, companheirismo e amizade nesses 4 anos de USP. Aos funcionários Rosendo, Gervásio, Felipe e Zico pelo cuidado no trato com os animais. Ao Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) pela concessão da bolsa de mestrado processo nº 169507/2017-3. Aos animais, nossos amados cavalos, criaturas apaixonantes, que se comportaram muito bem durante as coletas e involuntariamente auxiliaram na realização do projeto. ABSTRACT JARAMILLO, F. M. Evaluation of the protective effect of Cynara scolymus and Silybum marianum on the toxicity of imidocarb dipropionate in horses. 2019. 77 f. Dissertation (Master in Science) -Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, 2019. Herbal medicines are marketed in liquid, solid or semi-solid forms obtained from standard extracts, normally prepared by maceration or distillation. Despite the vast use of herbal medicines around the world, few of them have been scientifically validated so far in order to prove clinical efficacy and assess their safety. The aim of the present study was to evaluate the hepatoprotective and hepatoregulatory effect of the association of Cynara scolymus (Artichoke) and Silybum marianum (Milk Thistle), against the use of imidocarb dipropionate. Ten crossbred horses with a mean age of five years and seropositive for Theileria equi were used. The animals were randomly divided into 2 groups of 5 animals. All animals received imidocarb dipropionate at a dose of 5 mg / kg / day for 3 consecutive days. The control group (CG) did not receive any complementary treatment. The treated group (GT) received treatment with the compound once a day for 30 days. The animals were evaluated by means of a physical examination, as well as complementary hematological exams (hemogram, liver and muscle biochemical evaluation) and histological exams of fragments obtained by transcutaneous liver biopsy. The results of physical evaluations and complementary tests were compared between the groups and submitted to statistical analysis. During the course of this study no animals demonstrated discomfort or behavior change during compound administration. The physiological constants remained within the normal values throughout the treatment. Regarding the baseline values, the animals that received the compound did not show significant changes in the values of red blood cells, hemoglobin, hematocrit and platelets (p> 0.05). There were significant changes in the values of VCM, HCM, CHCM and leukocytes (p <0.05). In the evaluation of the hepatic profile, GT increased incomparison to the initial values and GT increase in relation to the GC in the concentrations of GGT, AST and CK (p <0.05). The total direct and indirect bilirubin concentration did not change significantly between the groups 11 (GT and GC) or between moments within the groups (p> 0.05). The enzymes GGT and AST presented a statistical difference between GT and GC (p <0.05), being higher in the group that received the compound. In the histological evaluation of the hepatic samples, there was an increase in portal inflammation in both groups in comparison to the initial values, being smaller in GT compared to CG on day -3. Lobular inflammation was also found in GT and GC animals at different times of the experiment, and was almost completely decreased in GT on days 12 and 30. Steatosis foci were identified in GT animals only on day 12 but occurred at all times in the GC. Deposition of pigment was identified in GT animals on days -3 and 30, and in CG animals at all times. Hydroponic degeneration was identified in the GT and CG animals on day 12. It was concluded that the use of the compound did not cause clinical changes in the animals treated with imidocarb dipropionate. Although the hematological and biochemical values were not altered in the use of the compound, it was observed that the GT effectively demonstrated a lower intensity of the hepatic lesions during the protocol of controlled toxicity.