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Toxicology in Vitro
Allergic contact dermatitis is a delayed T-cell mediated allergic response associated with relevant social and economic impacts. Animal experiments (e.g. the local lymph node assay) are still supplying most of the data used to assess the sensitization potential of new chemicals. However, the 7th amendment to the EU Cosmetic Directive have introduced a testing ban for cosmetic ingredients after March 2013. We have developed and optimized a stable and reproducible in vitro protocol based on humandoi:10.1016/j.tiv.2015.03.021 pmid:25868915 fatcat:ekksb4dud5axrlhwui6yyvntfm