Biting Deterrent Activity of a Deet Analog, Two DEPA Analogs, and SS220 Applied Topically to Human Volunteers Compared with Deet Against Three Species of Blood-Feeding Flies: Table 1

Jerome A. Klun, Ashot Khrimian, Edgar Rowton, Matthew Kramer, Mustapha Debboun
2006 Journal of medical entomology  
An earlier in vitro screening of N, and N,Ndiethylphenylacetamide (DEPA) analogs showed that two DEPA analogs, N,␣,]acetamide, and one Deet analog, N,N,]benzamide, had biting-deterrent activities that were superior to Deet against Aedes aegypti (L.) and Anopheles stephensi Liston. In the current study, the three analogs and (1S,2ЈS)-methylpiperidinyl-3-cyclohexene-1-carboxamide (SS220) were applied topically to the skin of human volunteers at 24 nmol compound/cm 2 skin and compared with the
more » ... mpared with the activity of Deet at the same dose against biting by Ae. aegypti, An. stephensi, and Phlebotomus papatasi Scopoli females. SS220 proved to be as effective as Deet against Ae. aegypti and P. papatasi but more effective than Deet against An. stephensi. Contrary to the earlier in vitro tests, results with humans (in vivo testing) showed that neither of the DEPA analogs nor the Deet analog performed more effectively than Deet against the insects. The in vivo results showed that the analogs were not sufÞciently effective to warrant further development. Notably, in vivo and in vitro methods used in discovery of personal protection chemicals for human use against blood-feeding ßies can both be effective discriminating tools, but results obtained with the respective methods may not always agree. Ultimately, we surmise that in vivo testing methods with humans must be used to discriminate among compounds that superÞcially seem effective when screened using an in vitro method. Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number.
doi:10.1093/jmedent/43.6.1248 pmid:17162960 fatcat:etd62kei5jcnfk3wnvoc5pcfbi