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Switch from NP-specific IgG3 to IgG1 in the mouse hybridoma cell line S24/63/63
1982
Journal of Immunology
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We have isolated by fluorescence-activated cell sorting an IgG1 expressing class switch variant (S24-1/47) from the IgG3-producing mouse hybridoma cell line S24/63/63. Both Ig bind the hapten 4-hydroxy-3-nitro-phenylacetyl (NP) with approximately the same affinity and fine specificity and are idiotypically indistinguishable. The apparent m.w. is 50,000 for the gamma 1 and 51,000 for the gamma 3 chain. The genomic DNA of IgG3-producing S24/63/63 cells contains a 14-kb Eco RI restriction fragment
doi:10.4049/jimmunol.128.3.1217
fatcat:x46ypsfkmrefrd37jorufo7uku