Role of transposable elements in heterochromatin and epigenetic control

Zachary Lippman, Anne-Valérie Gendrel, Michael Black, Matthew W. Vaughn, Neilay Dedhia, W. Richard McCombie, Kimberly Lavine, Vivek Mittal, Bruce May, Kristin D. Kasschau, James C. Carrington, Rebecca W. Doerge (+2 others)
<span title="2004-07-22">2004</span> <i title="Springer Nature"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/drfdii35rzaibj3aml5uhvr5xm" style="color: black;">Nature</a> </i> &nbsp;
proteins were removed by washing the beads six times with high ionic strength buffer. Bound protein was eluted with electrophoresis sample buffer containing 1% SDS, 2.5% 2-mercaptoethanol, 0.063 M Tris-HCl (pH 6.8) and 15% glycerol, and resolved by 6% or 8% SDS-PAGE. Western blot analysis was carried out with antisera against mPER2 (ref. 1), NPAS2 (ref. 10) and GAPDH (Sigma). Bound antibodies were detected by Supersignal West Pico Chemiluminescent Substrate (Pierce). Cyanocobalamin (335 mM) was
more &raquo; ... added to the binding solution for competition studies.
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