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MLC Seq: De novo sequencing of full-length tRNA isoforms by mass ladder complementation
tRNAs can exist in distinct isoforms because of different chemical modifications, which confounds attempts to accurately sequence individual tRNA species using next generation sequencing approaches or to quantify different RNA modifications at specific sites on a tRNA strand. Herein, we develop a mass spectrometric (MS) ladder complementation sequencing (MLC-Seq), allowing for direct and simultaneous sequencing of full-length tRNA molecules, including those with low abundance. MLC-seq isdoi:10.1101/2021.05.22.445286 fatcat:dvj34qpb45f3xpq2fd33rxn7yy