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Ordered and Sequential Binding of DnaA Protein tooriC, the Chromosomal Origin ofEscherichia coli
1996
Journal of Biological Chemistry
DnaA protein of Escherichia coli acts in initiation of chromosomal DNA replication by binding specific sequences, termed DnaA boxes in the chromosomal origin, oriC. On binding, it induces a localized unwinding to create a structure recognized by other replication proteins that act subsequently in the initiation process. In this report, we examined the binding of DnaA protein to each of the DnaA boxes in oriC. By gel mobility shift assays, DnaA protein formed at least six discrete complexes. ATP
doi:10.1074/jbc.271.29.17035
pmid:8663334
fatcat:6bh457sih5eankoua5yc5t2zo4