Whole-genome analysis reveals the complex evolutionary dynamics of Kenyan G2P human rotavirus strains
Journal of General Virology
Although G2P rotaviruses are common causes of acute childhood diarrhoea in Africa, to date there are no reports on whole genomic analysis of African G2P strains. In this study, the nearly complete genome sequences of two Kenyan G2P strains, AK26 and D205, detected in 1982 and 1989 , respectively, were analysed. Strain D205 exhibited a DS-1-like genotype constellation, whilst strain AK26 appeared to be an intergenogroup reassortant with a Wa-like NSP2 genotype on the DS-1-like genotype
... onstellation. The VP2-4, VP6-7, NSP1, NSP3 and NSP5 genes of strain AK26 and the VP2, VP4, VP7 and NSP1-5 genes of strain D205 were closely related to those of the prototype or other human G2P strains. In contrast, their remaining genes were distantly related, and, except for NSP2 of AK26, appeared to originate from or share a common origin with rotavirus genes of artiodactyl (ruminant and camelid) origin. These observations highlight the complex evolutionary dynamics of African G2P rotaviruses. 3Present address: Gynae-Paed Medical Centre, Nairobi, Kenya. The GenBank accession numbers for the nucleotide sequences of the VP1-4, VP6-7 and NSP1-5 genes of human RV-A G2P strains D205 and AK26 are JF304915-JF304936, respectively. A supplementary figure comparing the deduced amino acid sequences of the VP7 genes of strains AK26 and D205 with those of other strains and a supplementary table of primers used for amplification of different genes of strains AK26 and D205 are available with the online version of this paper.