P2.026 Audit of Adherence to UK National Guideline For Management of Epididymo-Orchitis
C Whitfield, S Rodgers
2013
Sexually Transmitted Infections
methods for diagnosis of Neisseria gonorrhoeae and Trichomonas vaginalis infections. However, PCR was available for testing for Chlamydia trachomatis, N. gonorrhoeae, Mycoplasma genitalium, Gardnerella vaginalis, M. hominis and Ureaplasma urealyticum using Russia-produced diagnostic tests. Serology remained in use for diagnosis of chlamydial infection and trichomoniasis. No appropriate and complete quality assurance and control system was available. Conclusions In Tver, Russia, the detection of
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... several STI agents has to be optimised, and international evidence-based standards and appropriate quality management systems introduced. Beneficially, the laboratory diagnosis is further centralised, which makes it easier to implement appropriate international evidence-based STI guidelines. Evaluation of thE GEnital Microbiota in MEn and WoMEn usinG an autoMatEd systEM for analysis of MicroscoPy iMaGEs of WEt and stainEd sMEars Background In Russia, microscopy of the genital microbiota in both women and men are performed at laboratory, not at physician's office. Microscopy is a simple and cheap method, which, together with a clinical presentation, provides sufficient information for diagnosis of bacterial vaginosis, vulvovaginal candidiasis, trichomoniasis, cervicitis, urethritis. Improvement of microscopy diagnostic methods with the use of automated computerised system is important. Methods Clinical samples were obtained from 100 men and 150 women of reproductive age. In total, 150 vaginal, cervical and female urethral samples, 73 male urethral samples and 17 prostatic samples were analysed using microscopy of Gram and methylene blue stained preparations. The presence of polymorphonuclear leukocytes, lactobacilli, "clue" cells, yeasts, trichomonads, grammenegative diplococci was assessed. Vaginal samples were also assessed for bacterial vaginosis using the Nugent score. For analysis, image analyzer including a Biological Microscope MT5000 Series, Neiji Techno Co., Ltd (Japan), digital colour camera Progress CT3 and software UroGyn were used. Results In 17.33%, 11.33% and 2% of the vaginal samples, "clue" cells, yeasts and trichomonads were visualised by microscopy. Signs of cervicitis were detected in 2% of the women, in none of the female samples signs of urethritis were seen. In men, signs of urethritis were revealed in 3.45%, and prostatitis -in 29.4% of the samples. In none of the samples, Gram-negative diplococci were detected. There was complete agreement between the results obtained using the image analyzer and those obtained using traditional light microscopy. Conclusion An automated system for the analysis of images obtained by microscopy investigation of urogenital samples from women and men is easy to use, allows documentation of results and facilitates their interpretation. EtioloGy of rEsPiratory tract infEction in hiv/ aids PatiEnts at thE national hosPital of troPical disEasEs (nhtd) hanoi, viEtnaM
doi:10.1136/sextrans-2013-051184.0291
fatcat:wos7aexml5dp5ckjykmcamuwge