Faculty of 1000 evaluation for Lane-by-lane sequencing using Illumina's Genome Analyzer II [dataset]

Jonathan Scolnick
2013 F1000 - Post-publication peer review of the biomedical literature   unpublished
Next-generation sequencing has become an essential tool in molecular biology and has been successfully applied to a broad variety of experimental approaches. While several platforms for next-generation sequencing exist, Illumina's sequencing-by-synthesis on the Genome Analyzer II (GAII) and the HiSeq2000 is the most common. A key constraint of these sequencers is the need to run multiple lanes of samples with identical parameters as part of a single flowcell. Here we present a series of
more » ... tions to the Illumina Genome Analyzer II sequencer as well as a script generating tool that allow laboratories to run the GAII in a lane-by-lane manner. Any number of lanes can be run at a time. Repeated use of the same flowcell on multiple sequencing runs does not appreciably reduce the intensity, cluster density or accuracy of the run. These modifications will enable smaller scale experiments with unusual design parameters to be run routinely on the GAII.
doi:10.3410/f.718011106.793476898 fatcat:urjtv4zgfjfczdgqvzpuxorucq