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A blue-native gel electrophoresis system was combined with an in organello import assay to specifically analyse the location and assembly of two nuclear-encoded photosystem II (PSII) subunits. With this method we were able to show that initially the low molecular mass PsbW protein is not associated with the monomeric form of PSII. Instead a proportion of newly imported PsbW is directly assembled in dimeric PSII supercomplexes with very fast kinetics; its negatively charged Nterminal domain isdoi:10.1016/s0014-5793(02)02314-1 pmid:11904154 fatcat:zr5jm5f4krdclbcbedgwogb6hq