Compared with traditional cables, superconducting multi-stage cables have the natural advantages of greater transmission power and less energy loss, which have gradually attracted attention. However, conventional multi-stage cables are based on low temperature superconducting (LTS) technology and there is considerable scope for improvement in their performance. In this paper, a novel structure of the multi-stage high temperature superconducting (HTS) twisted cable prepared by the

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... d-square (3S) wire is proposed. The AC loss characteristics of the twisted cable are deeply studied by experiments and simulation. Through the experiment, the influence of the voltage-leads on the AC loss measurement accuracy is eliminated, and frequency dependent is shown in the AC loss of the twisted cable. Besides, the simulated value of AC loss is consistent with the experimental value, which verifies the accuracy of the simulation. The AC loss of twisted cable is only 20% of that of the thin strip model, which reveals its outstanding advantages in AC loss.