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miRCURY LNATM Universal RT microRNA PCR (Exiqon) v1 (protocols.io.utbewin)
[dataset]
2018
protocols.io
1 Performing an RNA dilution series can be used to determine that input amounts are within the linear range of the system, and to ensure a good level of signal, with no sign of inhibition. Increasing the RNA volume to increase signal levels can have the opposite effect and result in less signal due to carry-over of more inhibitory compounds into the RT-PCR reaction. Standard methods for measurement of the RNA yield and quality are inappropriate for use with biofluid samples. The presence of
doi:10.17504/protocols.io.utbewin
fatcat:7szr44dwdjbkzgc2mik7es7rge