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A COMBINATION OF IN SILICO SUBTRACTIVE AND REVERSE VACCINOLOGY APPROACHES REVEALS POTENTIAL VACCINE TARGETS IN Corynebacterium pseudotuberculosis

SYAIDA ANATI ABD RASHID, Universiti Teknologi MARA Shah Alam, MOHAMAD FAKHRI YAACOB, NABIHAH RAIHANAH TAJUL ANUAR, NURANISAH JOHARI, ALYA NUR ATHIRAH KAMARUZAMAN, NORFATIMAH MAOHAMED YUNUS, MOHD FAKHARUL ZAMAN RAJA YAHYA, Universiti Teknologi MARA Shah Alam, Universiti Teknologi MARA Shah Alam, Universiti Teknologi MARA Shah Alam, Universiti Teknologi MARA Shah Alam (+2 others)
2022 Journal of sustainability science and management  

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Corynebacterium pseudotuberculosis is responsible for caseous lymphadenitis (CLA) in ruminants, which contributes to significant economic loss to some countries. Although extensive efforts are in progress to develop vaccines against this pathogen, to date no effective vaccine has been developed. The present work identifies potential vaccine targets in C. pseudotuberculosis. A combination of in silico subtractive and reverse vaccinology approaches were employed to identify non-host homologous
more » ... ential C. pseudotuberculosis proteins, which also fulfilled at least two of the following criteria: i) transmembrane domain, ii) signal peptide and iii) antigenic epitope. Results demonstrated that a total of 18 non-host homologous essential C. pseudotuberculosis proteins were considered as potential vaccine targets, including lipoprotein signal peptidase, ABC transporter ATP-binding protein, phosphate ABC transporter permease PstA, phosphoglucosamine mutase, inner membrane protein translocase component YidC and C4-dicarboxylate transporter DctA. Further analysis showed the potential role of inner membrane protein translocase component YidC as a hub protein. Homology modelling of this protein yielded a good model based on related structures from the Protein Data Bank. The development of a protective vaccine against C. pseudotuberculosis using non-host homologous essential proteins may offer an advantage to control caseous lymphadenitis in ruminants.
doi:10.46754/jssm.2022.01.007 fatcat:tywoxgrw7ne4hczn42wbryhefe
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SYAIDA ANATI ABD RASHID, Universiti Teknologi MARA Shah Alam, MOHAMAD FAKHRI YAACOB, NABIHAH RAIHANAH TAJUL ANUAR, NURANISAH JOHARI, ALYA NUR ATHIRAH KAMARUZAMAN, NORFATIMAH MAOHAMED YUNUS, MOHD FAKHARUL ZAMAN RAJA YAHYA, Universiti Teknologi MARA Shah Alam, Universiti Teknologi MARA Shah Alam, Universiti Teknologi MARA Shah Alam, Universiti Teknologi MARA Shah Alam, Universiti Teknologi MARA Shah Alam, Universiti Teknologi MARA Shah Alam. "A COMBINATION OF IN SILICO SUBTRACTIVE AND REVERSE VACCINOLOGY APPROACHES REVEALS POTENTIAL VACCINE TARGETS IN Corynebacterium pseudotuberculosis." Journal of sustainability science and management 17.1 (2022) 99-109