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Conditions of carbonic anhydrase extraction and activity measurement should be adapted to plant species and to tissues of plants. Different extraction and reaction media have been compared in ten different tree species in order to define the best conditions for carbonic anhydrase activity measurement. Bias and variability of differences in measured values were analysed according to Bland and Altman method. A comparison of titrimetric method with the traditional electrometric method ofdoi:10.5433/2316-5200.2014v3n1p48 fatcat:kc35clcy3fcdfbsdg5mh7jonta