Cryo-EM structure of the mature and infective Mayaro virus at 4.4 Å resolution reveals new features of arthritogenic alphaviruses [article]

Helder Ribeiro Filho, Lais Durco Coimbra, Alexandre Cassago, Rebeca Paiva Froes Rocha, Antonio Claudio Padilha, Joao Victor da Silva Guerra, Luiza Leme, Daniela Barretto Barbosa Trivella, Rodrigo Villares Portugal, Paulo Sergio Lopes-de-Oliveira, Rafael Elias Marques
2020 bioRxiv   pre-print
Mayaro virus (MAYV) is an emerging arbovirus of the Americas that may cause a debilitating arthritogenic disease. The biology of MAYV is not fully understood and largely inferred from related arthritogenic alphaviruses. Here we present the structure of MAYV at 4.4 Å resolution, obtained from a preparation of mature, infective virions. MAYV presents typical alphavirus features and organization. Interactions between viral proteins that lead to particle formation are described together with a
more » ... ogether with a hydrophobic pocket formed between E1 and E2 spike proteins and conformational epitopes specific of MAYV. We also describe MAYV glycosylation residues in E1 and E2 that may affect MXRA8 host receptor binding, and a molecular ″″handshake″″ between MAYV spikes formed by N262 glycosylation in adjacent E2 proteins. The structure of MAYV is suggestive of structural and functional complexity among alphaviruses, which may be targeted for specificity or antiviral activity.
doi:10.1101/2020.11.06.371773 fatcat:dkmblhr5xzgtrkrxv7a6zgl4di