Antimicrobial Activity Spectrum, cDNA Cloning, and mRNA Expression of a Newly Isolated Member of the Cecropin Family from the Mosquito VectorAedes aegypti

Carl Lowenberger, Maurice Charlet, Jacopo Vizioli, Sofie Kamal, Adam Richman, Bruce M. Christensen, Philippe Bulet
1999 Journal of Biological Chemistry  
An antimicrobial peptide belonging to the cecropin family was isolated from the hemolymph of bacteriachallenged adult Aedes aegypti. This new peptide, named cecropin A, was purified to homogeneity and fully characterized after cDNA cloning. The 34-residue A. aegypti cecropin A is different from the majority of reported insect cecropins in that it is devoid of a tryptophan residue and C-terminal amidation. The importance of these two structural features on the activity spectrum was investigated
more » ... m was investigated using a chemically synthesized peptide. A comparison of the antimicrobial activity spectrum of A. aegypti and Drosophila cecropin A showed a lower activity for the mosquito molecule. A. aegypti cecropin mRNA expression was not detected by Northern blot or reverse transcription-polymerase chain reaction analysis in any immature stage of the mosquito, nor in naïve adults, but it was observed in challenged adults 6 h after bacteria inoculation, and it continued over 7-10 days.
doi:10.1074/jbc.274.29.20092 pmid:10400619 fatcat:jvuj6gkpongyfdmxq74voyetxm