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Quantum dot (QD) imaging capability was investigated by the imaging depth at a near-infrared second optical window (SOW; 1000 to 1400 nm) using time-modulated pulsed laser excitations to control the effective fluence rate. Various media, such as liquid phantoms, tissues, and in vivo small animals, were used and the imaging depths were compared with our predicted values. The QD imaging depth under excitation of continuous 20 mW∕cm 2 laser was determined to be 10.3 mm for 2 wt% hemoglobin phantomdoi:10.1117/1.jbo.20.4.046012 pmid:25919424 fatcat:jtr6j5qesjhzvezadhppwyzmlm