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Immunoglobulin (Ig) was purified from the serum of eel (Anguilla japonica) and an indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of eel antibody. Sera were collected from eel which had been injected with formalin-killed Edwardsiella tarda bacterin. A pool of the sera was precipitatedwith a 40% saturated ammonium sulfate solution , and then immunoglobulin was purified from the precipitates by gel-filtration and ion-exchange chromatography . The purified Igdoi:10.3147/jsfp.26.201 fatcat:g6dus26h7ra2xgdvp3zcat63m4