Indirect Enzyme-linked Immunosorbent Assay (ELISA) for the Detection of Eel Serum Antibody

Takaji Iida, Hiroyuki Yonekura, Masafumi Izumiyama, Hisatsugu Wakabayashi
1991 Gyobyo kenkyu - Fish pathology  
Immunoglobulin (Ig) was purified from the serum of eel (Anguilla japonica) and an indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of eel antibody. Sera were collected from eel which had been injected with formalin-killed Edwardsiella tarda bacterin. A pool of the sera was precipitatedwith a 40% saturated ammonium sulfate solution , and then immunoglobulin was purified from the precipitates by gel-filtration and ion-exchange chromatography . The purified Ig
more » ... sified in Freund's complete adjuvant was administered to a rabbit to prepare anti-eel Ig serum . Eel were immersed in the E. tarda bacterin at a density of 1 mg wet weight/ml for 1 h . Change in antibody titers in the vaccinated fish was determined by indirect ELISA . The titers of vaccinated fish were significantly higher than those of unvaccinated control fish .
doi:10.3147/jsfp.26.201 fatcat:g6dus26h7ra2xgdvp3zcat63m4