Quantitative Evaluation of Inflammatory Response in Rats Submitted to the Abdominal Wall Surgery Using Peritoneum of Paca Preserved in 300% Supersaturated Solution of Sucrose or 98% Glycerin

Leonardo Martins Leal, Alessandra Regina Scavone Ferreira, Tais Harumi de Castro Sasahara, Aliny Antunes Barbosa Lobo Ladd, Paola Castro Moraes, Márcia Rita Fernandes Machado
2018 Open Journal of Animal Sciences  
This study aimed to quantitative evaluate the implantation of paca's peritoneum, a new biomaterial option, preserved in 300% supersaturated sugar solution or 98% glycerin on the abdominal wall of Wistar rats. 60 males, weighing between 150 and 200 g, are organized into the following experimental groups: control group (GI); group peritoneum preserved in 300% supersaturated sugar solution (GII); and group peritoneum preserved in 98% glycerin (GIII). Each group had 20 animals. Five rats from each
more » ... ive rats from each group underwent euthanasia at four different moments: 7 th , 15 th , 30 th , and 60 th post-operatory day for histological and stereological evaluations of the graft/native tissue interface. On histological examination, one found out an extensive presence of inflammatory infiltrate at the early periods (7 th and 15 th day) and an extensive presence of connective tissue at the late moments (30 th and 60 th day). Through stereological analysis, one found out that the number of mononuclear cells decreased throughout the evaluation moments. One concluded that the paca's peritoneum as a biological membrane preserved in 98% glycerin, when implanted on the abdominal wall of rats, microscopically allowed one to obtain, through stereological analyses, a lower inflammatory response than the paca's peritoneum preserved in 300% supersaturated sugar solution.
doi:10.4236/ojas.2018.84033 fatcat:6jyokk74zfduvfgvyh2si2vjey