20th ECP

2005 Virchows Archiv  
Introduction, Aims. The authors made a preliminary assessement of possible correlations between the amount of fibrillary components and capillary network of intratumoral stroma and the degree of glandular formation in 20 cases of invasive ductal carcinoma of the breast. Material and Methods. The samples were fixed in buffered formalin and included in paraffin wax. Stromal components were marked silver staining (Gömöri) and antibodies for CD34 Classs I. Five fields with no necrosis were selected
more » ... randomly using x10 objective, for each case, from the most representative slide for the tumoral pattern. The selected tumoral areas were aquired using a Nikon DN100 digital camera and a LuciaNet 1.16.2 soft. The quantitative determinations were performed, after image calibration, with analySIS Pro 3.2 soft. The 100 selected fields were subdivided in three groups following tubule formation: group I (score 1), group II (score 2), group III (score 3). The studied parameters for each group were: area occupied by stromal fibrillary elements, area occupied by malignant cells and capillary densities related to tumoral area, to malignant cells area and to stromal fibrillary components. Results. The group with less than 10% tubule formation (score 3)on the field area was the most numerous, followed by the group with score 2. Fibrilary component represented between 27,6% (group II) and 30% (group III), with a Confidence Level (95%) between 4,52 (group I) and 3,66 (group III). Vascular density related to whole tumoral area ranged between 39/mm2 (group I) and 43/mm2 (group II) with a Confidence Level (95%) between 8,72 (group I) and 4,68 (group III). Vascular density related to epithelial malignant cells was higher (around 66/mm2) in groups II and III, with a Confidence Level (95%) between 8,18 (group II) and 7,1 (group III). Vascular density related to stromal fibrillary components had its highest value in third group (191/mm2) but with large intervals of range in all three groups. Conclusions. These preliminary data showed no significant variations of the intratumoral stroma and capillaries amounts in different areas of ductal invasive breast carcinomas and also no significant correlation between stromal components variations and the degree of tubule formation.
doi:10.1007/s00428-005-1288-1 fatcat:25f45fdp5ffvdlo25zaqkddywm