RBPj -dependent Notch function regulates Gata2 and is essential for the formation of intra-embryonic hematopoietic cells

A. Robert-Moreno
2005 Development  
Aml1/Runx1, Gata2 and Scl/Tal1. We show that in wild-type embryos, a few cells lining the aorta endothelium at E9.5 simultaneously expressed Notch1 and Gata2, and demonstrate by chromatin immunoprecipitation that Notch1 specifically associated with the Gata2 promoter in E9.5 wild-type embryos and 32D myeloid cells, an interaction lost in RBPjκ mutants. Consistent with a role for Notch1 in regulating Gata2, we observe increased expression of this gene in 32D cells expressing activated Notch1.
more » ... ctivated Notch1. Taken together, these data strongly suggest that activation of Gata2 expression by Notch1/RBPjκ is a crucial event for the onset of definitive hematopoiesis in the embryo.
doi:10.1242/dev.01660 pmid:15689374 fatcat:bxmo4al4hnhrrksrque6abjfly