Hadamard-transform fluorescence-lifetime imaging

Takahiko Mizuno, Tetsuo Iwata
2016 Optics Express  
We discuss a Hadamard-transform-based fluorescence-lifetimeimaging (HT-FLI) technique for fluorescence-lifetime-imaging microscopy (FLIM). The HT-FLI uses a Fourier-transform phase-modulation fluorometer (FT-PMF) for fluorescence-lifetime measurements, where the modulation frequency of the excitation light is swept linearly in frequency from zero to a specific maximum during a fixed duration of time. Thereafter, fluorescence lifetimes are derived through Fourier transforms for the fluorescence
more » ... r the fluorescence and reference waveforms. The FT-PMF enables the analysis of multi-component samples simultaneously. HT imaging uses electronic exchange of HT illumination mask patterns, and a high-speed, highsensitivity photomultiplier, to eliminate frame-rate issues that accompany two-dimensional image detectors. 2016 Optical Society of America OCIS codes: (300.6280) Spectroscopy, fluorescence and luminescence; (180.2520) Fluorescence microscopy; (170.3650) Lifetime-based sensing. 16. K. Hassler, T. Anhut, and T. Lasser, "Time-resolved Hadamard fluorescence imaging," Appl. Opt. 44, 7564-7572 (2005). 17. D. Magde, R. Wong, and P. G. Seybold, "Fluorescence quantum yields and their relation to lifetimes of rhodamine 6G and fluorescein in nine solvents: Improved absolute standards for quantum yields," Photochem.
doi:10.1364/oe.24.008202 pmid:27137259 fatcat:5jeualf3hne6vl63ob7i4zdtxa