A recombinant antibody toolbox for Dictyostelium discoideum

Wanessa C. Lima, Philippe Hammel, Pierre Cosson
2020 BMC Research Notes  
The amoeba Dictyostelium discoideum has been a valuable model organism to study numerous facets of eukaryotic cell biology, such as cell motility, cell adhesion, macropinocytosis and phagocytosis, host-pathogen interactions and multicellular development. However, the relative small size of the Dictyostelium community hampers the production and distribution of reagents and tools, such as antibodies, by commercial vendors. For the past 5 years, our laboratory has worked to promote an increased
more » ... of recombinant antibodies (rAbs) by academic laboratories. Here we report our efforts to ensure that Dictyostelium researchers have access to rAbs. Using hybridoma sequencing and phage display techniques, we generated a panel of recombinant antibodies against D. discoideum antigens, providing a useful and reliable set of reagents for labelling and characterization of proteins and subcellular compartments in D. discoideum, accessible to the entire Dictyostelium community.
doi:10.1186/s13104-020-05048-8 pmid:32276653 fatcat:xvy75honrfdwhkuz4jx2zb2nta