The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

Rebeca Miranda-Castro, Ilaria Palchetti, Noemí de-los-Santos-Álvarez
2020 Frontiers in Chemistry  
Latest technological advancement has tremendously expanded the knowledge on the composition of body fluids and the cancer-associated changes, which has fueled the replacement of invasive biopsies with liquid biopsies by using appropriate specific receptors. DNA emerges as a versatile analytical reagent in electrochemical devices for hybridization-based or aptamer-based recognition of all kind of biomarkers. In this mini review, we briefly introduce the current affordable targets (tumor-derived
more » ... ucleic acids, circulating tumor cells and exosomes) in body fluids, and then we provide an overview of selected electrochemical methods already applied in clinical samples by dividing them into three large categories according to sample type: red (blood), yellow (urine), and white (saliva and sweat) diagnostics. This review focuses on the hurdles of the complex matrices rather than a comprehensive and detailed revision of the format schemes of DNA-based electrochemical sensing. This diverse perspective compiles some challenges that are often forgotten and critically underlines real sample analysis or clinical validation assays. Finally, the needs and trends to reach the market are briefly outlined.
doi:10.3389/fchem.2020.00143 pmid:32266206 pmcid:PMC7099045 fatcat:dln4iq4unvb2jfswzwmotndb7a