Isolation of Cancer Epithelial Cells from Mouse Mammary Tumors

Sara Johnson, Hexin Chen, Pang-Kuo Lo
2013 Bio-protocol  
The isolation of cancer epithelial cells from mouse mammary tumor is accomplished by digestion of the solid tumor. Red blood cells and other contaminates are removed using several washing techniques such that primary epithelial cells can further enriched. This procedure yields primary tumor cells that can be used for in vitro tissue culture, fluorescence-activated cell sorting (FACS) and a wide variety of other experiments (Lo et al., 2012).
doi:10.21769/bioprotoc.326 fatcat:dqlnctiswzagdbba267ijgr2em