Increased Resistance to Quinolones in Streptococcus parauberis and Development of a Rapid Assay for Detecting Mutations in Topoisomerase Genes
Streptococcus parauberis의 퀴놀론 내성 증가와 Topoisomerase 유전자에서의 돌연변이 신속 분석

So Yeon Kim, Young Chul Kim, Seo Kyung Jeong, Lyu Jin Jun, Ji Woong Jin, Hyun Do Jeong
2014 Korean Journal of Fisheries and Aquatic Sciences  
To investigate the acquisition of quinolone resistance, we examined mutations in the quinolone resistance-determining region (QRDR) of type II topoisomerase genes in ciprofloxacin (CIP)-resistant clinical isolates and in vitro mutants of Streptococcus parauberis. The CIP-resistant clinical isolates had one base change responsible for a Ser-79→Thr in the QRDR of parC. However, the CIP-resistant in vitro mutants had an altered QRDR of parC (Ser-79→Ile) that differed from that of the isolates.
more » ... of the CIP-resistant S. parauberis clinical isolates or in vitro mutants exhibited amino acid changes in gyrA or gyrB. However, even though involvement in the increased resistance was not clear, an Arg-449→Ser mutation outside of the QRDR of parE was detected in CIP-resistant mutant 2P1. These results suggest that the topoisomerase IV gene, parC (and possibly parE, as well), is the primary ciprofloxacin target in S. parauberis. Additionally we established a high-resolution melting (HRM) assay capable of detecting the dominant mutation in four type II topoisomerase genes conferring ciprofloxacin resistance. These rapid and reliable assays may provide a convenient method of surveillance for genetic mutations conferring antibiotic resistance.
doi:10.5657/kfas.2014.0247 fatcat:wq6yzyiitnghnavc7ecuw4ueve