al.13 ) have reported that the intradermal growth of syngeneic hepatocarcinoma (line 10) in normal strain 2 guinea pigs was suppressed when the tumor cells are inoculated together with living cells of Mycobacterium bovis BCG strain. Tanaka and Tokunaga10) have shown similar results in mice using syngeneic and allogeneic tumor cells. Recently, it was shown that oil-attached cell wall or cell wall skeleton of BCG are as effective as live BCG in the suppression of line 10 hepatocarcinoma in strain

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... 2 guinea pigs.8,14) This paper reports that three kinds of oil-attached mycobacterial fractions, whole cell wall, cell wall skeleton, and peptidoglycolipid, have potent tumor suppression and regression activities in syngeneic and allogeneic tumors, such as sarcoma-180, mastocytoma-P815, and EL-4 leukemia, in mice. Whole cell wall and cell wall skeleton were prepared by the method described previously.4) The cells of BCG (Pasteur Institute strain) were disrupted in a Sorvall RF-1 cell fractionator at 35,000lb/in2 at 5-10°. The disrupted product