Due to the constant extractivism and changes in the environment, destruction of the habitats of orchids, many species have been extinct, and some others are on a near extinction list. Some species have economic importance, such as vanilla, and others with medicinal properties and some with ornamental value, due to the beauty of its flowers and variety of species, being used in the landscaping. An alternative for the preservation of these species would be through the germplasm bank.

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... ion in liquid nitrogen is a widely studied and diffused method in several areas. Several protocols are studied with the purpose of reducing the rate of deterioration after these storage procedures and thus increasing the storage time of the seeds with quality. Therefore, this work aims to present intra and extracellular cryoprotectants found in the literature for cryopreservation of orchids. However, in order to obtain quality materials, the type and type of cryopreserved material and storage time must be considered.