Determination of free and bound manganese(II) in hepatocytes from fed and fasted rats

D E Ash, V L Schramm
1982 Journal of Biological Chemistry  
Free and total Mn(II) content of hepatocytes from fed and fasted rats has been determined by EPR. The difference spectra of cumulative scans of cell samples and the mother medium allows a sensitivity of 0.1 PM free Mn(II). The free and total Mn(II) content was 0.71 2 0.10 and 34.4 f 4.4 nmol/ml of cell H 2 0 in hepatocytes from fed animals and was 0.25 -+, 0.15 and 35.9 +. 7.6 nmol/ml of cell HzO, respectively, in hepatocytes from fasted animals. These results demonstrate a 2.8-fold decrease in
more » ... free Mn(I1) with no change in the total cell Mn(II) in response to fasting.
pmid:6286611 fatcat:qnk4c47scnffpgylxwup55tdxm