Chemoenzymatic Synthesis of Classical and Non-classical Anticoagulant Heparan Sulfate Polysaccharides

Balagurunathan Kuberan, David L. Beeler, Miroslaw Lech, Zhengliang L. Wu, Robert D. Rosenberg
2003 Journal of Biological Chemistry  
Heparan sulfate (HS) polysaccharides interact with numerous proteins at the cell surface and orchestrate many different biological functions. Though many functions of HS are well established, only a few specific structures can be attributed to HS functions. The extreme diversity of HS makes chemical synthesis of specific bioactive HS structures a cumbersome and tedious undertaking that requires laborious and careful functional group manipulations. Now that many of the enzymes involved in HS
more » ... ynthesis are characterized, we show in this study how one can rapidly and easily assemble bioactive HS structures with a set of cloned enzymes. We have demonstrated the feasibility of this new approach to rapidly assemble antithrombin III-binding classical and non-classical anticoagulant polysaccharide structures for the first time.
doi:10.1074/jbc.m305029200 pmid:14519763 fatcat:ysnm4quskze4zp4fdfu2awv5du