Multiscale modeling of influenza A virus replication in cell cultures predicts infection dynamics for highly different infection conditions

Daniel Rüdiger, Sascha Young Kupke, Tanja Laske, Pawel Zmora, Udo Reichl, Claus O. Wilke
2019 PLoS Computational Biology  
OPEN ACCESS Citation: Rüdiger D, Kupke SY, Laske T, Zmora P, Reichl U (2019) Multiscale modeling of influenza A virus replication in cell cultures predicts infection dynamics for highly different infection conditions. PLoS Comput Biol 15(2): e1006819. https://doi. conditions in closed systems, better scalability, and a short response time in case of pandemic outbreaks. Here, we employ a computational model to describe underlying mechanisms during the IAV infection in adherent MDCK cells.
more » ... attention was paid on the influence of the MOI on virus spread in cell populations. Although dynamics between infections with high and low amounts of infecting virions differ significantly, our model closely captures both scenarios. Furthermore, our results provide insights into IAVinduced apoptosis and the switch from transcription to replication in intracellular IAV replication. Additionally, model simulations indicate how virus particle release is regulated, and what impact defective interfering particles have on virus replication in different infection conditions. Taken together, we developed a computational model that enables detailed analyses of IAV replication dynamics in animal cell culture. Predictive multiscale model of influenza A virus replication PLOS Computational Biology | https://doi.org/10.Predictive multiscale model of influenza A virus replication PLOS Computational Biology | https://doi.org/10. Fig 4. Implementation of an inhibition of viral mRNA synthesis improves description of viral mRNA dynamics. (A) Model fits to viral mRNA measurements of MDCK cultures infected with influenza A/PR/8/34 (H1N1) at an MOI of 73 based on TCID 50 [4]. Error bars indicate standard deviations of three individual experiments. (B) Percentage of viral mRNA synthesis rate for different model versions, experimental data were adapted from [27]. In short, BHK-21 cells were infected by influenza virus (WSN strain) at 10 to 20 PFU per cell. Predictive multiscale model of influenza A virus replication PLOS Computational Biology | https://doi.org/10.
doi:10.1371/journal.pcbi.1006819 fatcat:cxeoz7sfijanpowlpriqoxxe5m