Objective: To explore cisplatin-induced apoptosis and proliferation inhibition effect of Hep-2 cells following the interference of livin expression in laryngeal carcinoma, and to explore the mechanism of livin in the drug resistance of laryngeal carcinoma cell line Hep-2. Methods: Different concentrations of cisplatin were acted on laryngeal carcinoma Hep-2 cells. The MTT assay was used to detect changes of laryngeal carcinoma cells on the proliferation inhibitory sensitivity of cisplatin

more »

... ing the exhibition of livin expression. The apoptosis rate was detected after laryngeal carcinoma cell line was treated by cisplatin for 48h, and the change of apoptotic factor capase-3 activity was detected. Results: 1.MTT assay results showed that by interfering livin gene expression, at the same cisplatin concentrations, the proliferation inhibition rate of cells in the group Hep2-pLivin was significantly higher than that of the Hep2-pHK (P<0.05) 2. After inhibiting the expression of livin gene in laryngeal carcinoma cells, the activity of caspase-3 in cells might be enhanced, and the activation effect was further enhanced after the addition of cisplatin. Conclusion : Targeting inhibition of the expression of livin gene can enhance the apoptosis of laryngeal carcinoma cells and improve the sensitivity to cisplatin. Wang SL (2018) Inhibition of livin gene combined with cisplatin affect the apoptosis of laryngeal carcinoma Hep-2 cells by RNA interference technique Volume 3(3): 2-5