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When significant mortality of the bathyal spatangoid echinoid Paleopneustes cristatus occurred under laboratory conditions, we investigated the cause and course of the disease by culturing and identifying internal pathogens, then experimentally infecting healthy urchins with isolates of the suspected disease organism. The pathogen was determined to be the Gram-negative halophilic bacterium Vibrio alginolyticus. This species was also recovered from frozen post-challenge specimens of P. cristatusdoi:10.3354/dao039193 pmid:10768287 fatcat:sxvjnp7yarbo7g752mtxkfb3n4