The present investigation comprising of 101 barley genotypes was conducted at Genetics and Plant Breeding, Banaras Hindu University, during rabi of 2016-17. Variability and diversity analysis was carried out based on data collected on 14 various quantitative traits. Analysis of variance revealed that there was a significant difference among the sixty four genotypes for all the characters studied. The phenotypic coefficients of variation (PCV) values were higher than genotypic coefficients of

more »

... iation (GCV) values for all the traits studied. Medium phenotypic coefficients of variation (PCV) and genotypic coefficients of variation (GCV) were recorded for days to 50% flowering, effective tillers per plant, spike length with awn, spike length without awn and plant height. Medium phenotypic coefficients of variation (PCV) and low genotypic coefficients of variation (GCV) values were displayed for SPAD value and leaf rolling. Low phenotypic coefficients of variation (PCV) and genotypic coefficients of variation (GCV) values were recorded for days to maturity which suggests the limitation of selection for these traits. High heritability values were observed in all the characters studied except leaf rolling. The expected genetic advance as a percent of mean ranged from 3.45 (Leaf rolling) to grain yield per plant (77.14). Characters with a high genetic advance as a percent of mean allow the improvement of this character through selection Genetic diversity was assessed in 101 genotypes of barley by using D2 analysis. The genotypes were grouped into 11 clusters. Cluster II comprised 40 genotypes which was followed by clusterI. Cluster with small statistical distances considered less diverse than those with large distances. The intra cluster value was maximum in cluster X and minimum in cluster I. Cluster V showed the highest mean values for days to maturity, effective tillers per plant, stomatal conductivity, grain per ear, yield per plant while cluster V revealed the lowest mean value for leaf rolling. The characters responsible for genetic divergence days to maturity, effective tillers per plant, stomatal conductivity, grain per ear, yield per plant.