Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania

Patricia Manuela Vieira, Narcisa Mederle, Maria Luisa Lobo, Kalman Imre, Ovidiu Mederle, Lihua Xiao, Gheorghe Darabus, Olga Matos
2015 Folia Parasitologica  
To investigate the transmission of species of Cryptosporidium Tyzzer, 1907 in Timis County, Romania, 48 isolates of Cryptosporidium coccidia from 11 children, 29 calves and eight pigs were characterised by molecular analysis of two loci (SSU rRNA and 60-kDa glycoprotein gene). Overall, 22 isolates were amplified and sequence analyses revealed that all isolates were Cryptosporidium parvum Tyzzer, 1912. Two subtype families were identified, IIa and IId. Subtype IIdA22G1 (n = 4) was the single C.
more » ... was the single C. parvum subtype found in children. Subtypes found in calves included IIdA27G1 (n = 8), a novel subtype, IIdA25G1 (n = 5), IIdA22G1 (n = 2), IIdA21G1a (n = 1), and IIaA16G1R1 (n = 1). Subtype IIdA26G1 was found in a pig. These results were significantly different from previous Romanian reports, as the five subtypes of family IId identified in this study were never identified previously in this country. Thus, cattle may be a source of Cryptosporidium infections for humans and the transmission dynamics of C. parvum in Romania is more complex than previously believed. Cite this article as: Vieira P.M., Mederle N., Lobo M.L., Imre K., Mederle O., Xiao L., Darabus G., Matos O. 2015: Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania. Folia Parasitol. 62: 002.
doi:10.14411/fp.2015.002 pmid:25960546 fatcat:faginybb6fbjjdtjk7hmynnxfi