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Escherichia coli cells that produce only plasmid-encoded wild-type or mutant GroEL were generated by bacteriophage P1 transduction. Effects of mutations that affect the allosteric properties of GroEL were characterized in vivo. Cells containing only GroEL(R197A), which has reduced intra-ring positive cooperativity and inter-ring negative cooperativity in ATP binding, grow poorly upon a temperature shift from 25 to 42°C. This strain supports the growth of phages T4 and T5 but not phage anddoi:10.1074/jbc.m007594200 pmid:10973985 fatcat:xzi2yyyfmze2dnq3sszptmqndm