Lipid Droplet Biogenesis Induced by Stress Involves Triacylglycerol Synthesis That Depends on Group VIA Phospholipase A2

Albert Gubern, Miquel Barceló-Torns, Javier Casas, David Barneda, Roser Masgrau, Fernando Picatoste, Jesús Balsinde, María A. Balboa, Enrique Claro
2008 Journal of Biological Chemistry  
This work investigates the metabolic origin of triacylglycerol (TAG) formed during lipid droplet (LD) biogenesis induced by stress. Cytotoxic inhibitors of fatty acid synthase induced TAG synthesis and LD biogenesis in CHO-K1 cells, in the absence of external sources of fatty acids. TAG synthesis was required for LD biogenesis and was sensitive to inhibition and down-regulation of the expression of group VIA phospholipase A 2 (iPLA 2 -VIA). Induction of stress with acidic pH, C 2 -ceramide,
more » ... C 2 -ceramide, tunicamycin, or deprivation of glucose also stimulated TAG synthesis and LD formation in a manner dependent on iPLA 2 -VIA. Overexpression of the enzyme enhanced TAG synthesis from endogenous fatty acids and LD occurrence. During stress, LD biogenesis but not TAG synthesis required phosphorylation and activation of group IVA PLA 2 (cPLA 2 ␣). The results demonstrate that iPLA 2 -VIA provides fatty acids for TAG synthesis while cPLA 2 ␣ allows LD biogenesis. LD biogenesis during stress may be a survival strategy, recycling structural phospholipids into energy-generating substrates.
doi:10.1074/jbc.m806173200 pmid:19117952 fatcat:5xhqpxvn7vcabhdajl55br7zkm