Corticotropin-Releasing Hormone Receptor Type 1 and Type 2 Mediate Differential Effects on 15-Hydroxy Prostaglandin Dehydrogenase Expression in Cultured Human Chorion Trophoblasts

Lu Gao, Ping He, Jinyan Sha, Chunmin Liu, Ling Dai, Ning Hui, Xin Ni
2007 Endocrinology  
Throughout gestation, the chorion laeve controls the levels of biologically active prostaglandins (PGs) by its high level of nicotinamide adenine dinucleotide-dependent 15-hydroxy PG dehydrogenase (PGDH). In this study, we investigate the effects mediated by CRH receptors on the expression of PGDH in the chorion. We found that both CRHR1 and CRHR2 were localized in cultured chorion trophoblast cells, with CRH-R1␣, R1␤, R1c, R1e, and R1f and CRHR2␤ isoforms identified in these cells. To block
more » ... cells. To block the actions of endogenous CRH and its related peptides, cultured chorion trophoblasts were treated with an increasing concentration of ␣-helical CRH 9 -41, the nonselective CRH receptor antagonist, which resulted in decreased mRNA and protein expression as well as the activity of PGDH. To investigate the individual role of CRHR1 and CRHR2, cell cultures were treated with the specific CRHR1
doi:10.1210/en.2006-1212 pmid:17463062 fatcat:tn6yvxrx5bbsnjxa3fuegifmgy