DNA fingerprinting of Plectranthus plants for protection of cultivar registration

L.J. Rice, G.D. Ascough, J.F. Finnie, J. Van Staden
2010 South African Journal of Botany  
protease inhibitor to enhance production of the VP1 antigenic protein. The VP1 construct was infiltrated into the plant through the method of syringe agro-infiltration. The VP1 protein was then transiently expressed in the cytosol of non-transgenic tobacco plants (N. tabacum) as well as in transgenic tobacco plants expressing the oryzacystatin and co-expressing a maize serine protease inhibitor. We are currently using SDS-PAGE analysis to analyse the protein profile and Western blotting to
more » ... rn blotting to confirm the presence and amount of expression of VP1 in the different types of tobacco plants. First data about the different analyses will be presented.
doi:10.1016/j.sajb.2010.02.040 fatcat:tjzm5qytkbc6pc2hp3uwzfbzeu