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A double-antibody radioimmunoassay for PMSG, especially for measuring PMSG in cattle blood after exogenous application, has been developed. A rabbit antiserum against PMSG and pure PMSG for radioiodination were used. There was a strong cross-reaction against equine LH and FSH, but the slight cross-reaction against bovine LH and FSH could be eliminated by adding bovine LH to each tube during the assay. Unspecific, interfering influences of equine or cow serum could be eliminated by adding adoi:10.1530/jrf.0.0550339 pmid:439068 fatcat:rj4zpah7fndevhkklyywvdpwdm