Microspore culture method can be used as plant breeding program. The preparation of cultivars selection is an important step. The research starts with cultivated five cultivars of soybean i.e: Argomulyo, Grobogan, Wilis, Anjasmoro and Black Malika. The appearence of soybean plant was observed until flowering. Selection of plants based on: sum of flower bud every plant, anther midline, total and diameter of microspore every flower bud was measured by the 'Optilab' software. The development of

more »

... rospore done with grouping of flower bud according long 2.02.5 mm, 2.6-3.0 mm, 3.1- 3.6 mm and 3.7-4.1 mm for chooses flower bud with the most late uninucleate microspore stadium. Result of the research shows that long of flower bud 2.6-3.6 mm contain 1847-2010 late uninucleate microspores, diameter 20 µm for 5 cultivars can be used for material of microspore culture. Anjasmoro cultivar, tall of plant gain 68 cm, sum of rame 7-9, anther midline 354.67±59.67 µm, number of microspores each flower bud 2003±216. Result of responsive qulity test with anther incubation on 340C temperature for 4 days represent the most of total viable microspore, 3.371±45 on Anjasmoro cultivar. Plant breeding by Anjasmoro cultivar is the most appropriate for microspore culture treatment.