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Ammonium and methylammonium transport by the nitrogen-fixing bacterium Azotobacter vinelandii
Journal of Bacteriology
Azotobacter vinelandii, grown with NH4' as nitrogen source, was shown to possess an active transport system which can take up NH4' against a concentration gradient of 58-fold. The properties of the NH4' uptake system were investigated with the NH4' analog CH3NH3'. The use of this analog was justified on the basis of the conclusion that the uptake of NH4+ and CH3NH3+ involves a common binding site, as shown by the competitive inhibition of CH3NH3+ uptake by NH4+ (Ki approximately 3 ,uM). Adoi:10.1128/jb.148.2.435-442.1981 fatcat:tnheiimekbcldjn24o6fx4vibu