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Automated phenol-chloroform extraction of high molecular weight genomic DNA for use in long-read single-molecule sequencing
[article]
2022
bioRxiv
pre-print
In order to automate the genome sequencing pipeline in our laboratory, we programmed a dual-arm anthropomorphic robot, the Robotic Biology Institute's Maholo LabDroid, to perform organic solvent-based genomic DNA extraction from cell lysates. To the best of our knowledge, this is the first time that automation of phenol-chloroform extraction has been reported. We achieved routine extraction of high molecular weight genomic DNA (>100 kb) from diverse biological samples including algae cultured
doi:10.1101/2022.01.26.477939
fatcat:lvkq2qmfbjfchguorlhpd7m4ya