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Cellular distribution of activity for three enzymes with maltose binding protein as fusion partner and the structural implications
Turkish journal of chemistry
The bacterial SEC pathway is commonly used for secretion of heterologous proteins in E. coli by fusing them to transported proteins to facilitate downstream processing. While some proteins are translocated very efficiently, some reside in the cytoplasm. In this work, maltose binding protein (MBP) was fused to 3 cytoplamic enzymes from Thermus thermophilus (serine protease, 251 residues; glucose isomerase, 381 residues; pullulanase, 718 residues) to study the protein transport from the cytoplasmdoi:10.3906/kim-0901-37 fatcat:prc46suvnnafpaueqn2us6vq4m