Glutathione-Dependent Enzymes and Glutathione in Infertility of Men with Different Body Mass
Глутатионзависимые ферменты и глутатион при бесплодии мужчин с различной массой тела

L.I. Kolesnikova, O.A. Vanteeva, N.A. Kurashova, B.G. Dashiev
2015 Rossiiskaya Akademiya Meditsinskikh Nauk. Vestnik  
Objective: Our aim was to investigate the glutathione-disulfide system of antioxidant defense in men with different body mass index and infertility. Methods: 60 men with infertility were examined -30 with normal weight and 30 with overweight. 30 fertile men were included in the control group. Hemolysate made from red blood cells was used as a material for study. The level of reduced (GSH) and oxidized glutathione (GSSG), and glutathione activity was determined by enzyme spectrofluorophotometer
more » ... rofluorophotometer SHIMADZU-1501 (Japan). Results: In infertile men with normal body weight decrease of GSH to 15.35% was combined with a decrease in the activity of enzymes -glutathione reductase (GR) to 53.41%, glutathione peroxidase (GPO) to 51.44%, and increased activity of glutathione-S-transferaze (GST) to 31.52% as compared with the control group. In the group of infertile men with overweight a decrease in the activity of GR, 56.77% and 56.22% at the GPO on the background of a stable level of GST was detected in comparison with fertile men. The concentration of GSH in infertile group decreased by 20.08%, and the concentration of GSSG increased by 20.38%. Conclusion: It has been established that imbalance in the glutathione system is the most prominent feature in men with infertility and overweight as compared to infertile men with normal body weight.
doi:10.15690/vramn.v70i1.1226 fatcat:kpwblkmzbfhmhdcaogysyrze4q